Journal: bioRxiv

Article Title: A novel Gorilla-derived oncolytic Adenovirus with natural selective replication in cancer cells

doi: 10.64898/2026.02.26.708271

Figure Lengend Snippet: (A) A549 cells were infected with GRAd32 or GRAd25 at MOI 50 in the presence (w) or absence (w/o) of an anti-CD46 blocking antibody. Images were acquired 3 days post-infection. Competition with CD46 resulted in a delayed onset of cytopathic effect (CPE) exclusively in GRAd25-infected cells. (B) Flow cytometry analysis of surface CAR and CD46 expression levels in lung cancer (A549, NCI-H727, NCI-H1975, NCI-H1299) and normal (MRC5, HUVEC) cell lines. (C) Infection of MRC5 and A549 cells with a replication-defective GRAd32 GFP reporter virus. GFP expression was evaluated at 48 hours post-infection. The presence of GFP signal confirms that GRAd32 is capable of entering MRC5 cells despite its inability to replicate in this cell line.

Article Snippet: For the evaluation of CD46 and CAR cell surface levels, 2-3 x 10 5 cells (MRC5, HUVEC, A549, NCI-H1299, NCI-H1975, NCI-H727) were collected and incubated with anti-human CD46 (1:50; mouse monoclonal; #12239-MM05, Sino Biological) or with anti-human CAR (1:50; rabbit monoclonal; #10799-R271, Sino Biological) for 30 min at 4°C.

Techniques: Infection, Blocking Assay, Flow Cytometry, Expressing, Virus

Journal: International journal of molecular sciences

Article Title: Peritoneal Expression of Membrane Complement Regulators Is Decreased in Peritoneal Dialysis Patients with Infected Peritonitis.

doi: 10.3390/ijms24119146

Figure Lengend Snippet: Figure 1. Correlations of expressions of membrane complement regulators (CRegs), CD46, CD55, and CD59, deposition of complement (C) activation products such as aC3 and C5b-9, and levels of sC5b-9 in peritoneal dialysis fluid (PDF) with tissue injury score and comparison of sC5-9 levels in PDF between Groups P and NP. Expressions of each of the CRegs CD46, CD55, and CD59 showed good inverse correlations with the severity score for peritoneal injuries ((A–C) respectively). In contrast, deposition of C activation products such as activated C3 (aC3) and C5b-9 correlated with the severity score of peritoneal tissue injures ((D,E) respectively). Adjusted PDF levels of sC5b-9 (sC5b-9/TP) also correlated with the severity score for peritoneal injures (F). Between Groups P and NP, the tissue injury score and PDF levels of sC5b-9/TP were significantly different ((G,H), respectively). Dots in the graph show outliers. Outliers were defined as values greater than the 75th percentile plus 1.5 times the interquartile range. #, p < 0.05.

Article Snippet: Monoclonal Ab (mAb) mouse antihuman CD46 (MEM 258) was purchased from BIO-RAD (Hercules, CA, USA).

Techniques: Membrane, Activation Assay, Comparison

Journal: International journal of molecular sciences

Article Title: Peritoneal Expression of Membrane Complement Regulators Is Decreased in Peritoneal Dialysis Patients with Infected Peritonitis.

doi: 10.3390/ijms24119146

Figure Lengend Snippet: Figure 4. Expressions of CRegs CD46, CD55, and CD59 in those with and without peritonitis and depositions of complement (C) activation products. Frames (A–E) and (F–J) represent peritoneal tissues in Group P and Group NP, respectively. Frames (A,F), frames (B,G), frames (C,H), frames (D,I), and frames (E,J) show expressions of CD46, CD55, and CD59 and depositions of activated C3 (aC3) and C5b- 9, respectively. Graphs (K–O) show results for expressions of CD46, CD55, and CD59 and deposition of aC3 and C5b-9, respectively. Original magnification of (A–C) and (F–H) is ×400, while that of (D,E,I,J) is ×100. Inserted frames in right upper corners of frames (F–H) are at double the original magnification. Scale bars of 50 µm and 200 µm are shown in the left upper corner of frames (A,D), respectively. * shows peritoneal cavity side. Dots in the graph show outliers. Outliers were defined as values greater than the 75th percentile plus 1.5 times the interquartile range. # p < 0.05.

Article Snippet: Monoclonal Ab (mAb) mouse antihuman CD46 (MEM 258) was purchased from BIO-RAD (Hercules, CA, USA).

Techniques: Activation Assay

Journal: International journal of molecular sciences

Article Title: Peritoneal Expression of Membrane Complement Regulators Is Decreased in Peritoneal Dialysis Patients with Infected Peritonitis.

doi: 10.3390/ijms24119146

Figure Lengend Snippet: Figure 5. Pathological changes with accumulation of inflammatory cells of peritonitis and expression of membrane complement regulators (CRegs) caused by Candida spp. or P. aeruginosa (Group P1) and Gram-positive cocci (Group P2). Graphs (A,B) show the tissue injury scores and the proportion of cytokeratin-positive mesothelial cells in the peritoneum, respectively. Graph (C–E) shows the numbers of esterase-positive neutrophils, CD3-positive pan-T cells, and CD68-positive macrophages, respectively. Graphs (F,G) show the scores of fibrin deposition and fibrosis in the peritoneum, respectively. Graphs (H–J) show CRegs CD46, CD55, and CD59, respectively. Group P1 shows cases with fungal infection or P. aeruginosa and Group P2 shows cases with Gram-positive cocci. Dots in the graph show outliers. Outliers were defined as values greater than the 75th percentile plus 1.5 times the interquartile range. #, p < 0.05.

Article Snippet: Monoclonal Ab (mAb) mouse antihuman CD46 (MEM 258) was purchased from BIO-RAD (Hercules, CA, USA).

Techniques: Expressing, Membrane, Infection

Journal: International Journal of Molecular Sciences

Article Title: Peritoneal Expression of Membrane Complement Regulators Is Decreased in Peritoneal Dialysis Patients with Infected Peritonitis

doi: 10.3390/ijms24119146

Figure Lengend Snippet: Correlations of expressions of membrane complement regulators (CRegs), CD46, CD55, and CD59, deposition of complement (C) activation products such as aC3 and C5b-9, and levels of sC5b-9 in peritoneal dialysis fluid (PDF) with tissue injury score and comparison of sC5-9 levels in PDF between Groups P and NP. Expressions of each of the CRegs CD46, CD55, and CD59 showed good inverse correlations with the severity score for peritoneal injuries (( A – C ) respectively). In contrast, deposition of C activation products such as activated C3 (aC3) and C5b-9 correlated with the severity score of peritoneal tissue injures (( D , E ) respectively). Adjusted PDF levels of sC5b-9 (sC5b-9/TP) also correlated with the severity score for peritoneal injures ( F ). Between Groups P and NP, the tissue injury score and PDF levels of sC5b-9/TP were significantly different (( G , H ), respectively). Dots in the graph show outliers. Outliers were defined as values greater than the 75th percentile plus 1.5 times the interquartile range. # , p < 0.05.

Article Snippet: Monoclonal Ab (mAb) mouse anti-human CD46 (MEM 258) was purchased from BIO-RAD (Hercules, CA, USA).

Techniques: Membrane, Activation Assay, Comparison

Journal: International Journal of Molecular Sciences

Article Title: Peritoneal Expression of Membrane Complement Regulators Is Decreased in Peritoneal Dialysis Patients with Infected Peritonitis

doi: 10.3390/ijms24119146

Figure Lengend Snippet: Expressions of CRegs CD46, CD55, and CD59 in those with and without peritonitis and depositions of complement (C) activation products. Frames ( A – E ) and ( F – J ) represent peritoneal tissues in Group P and Group NP, respectively. Frames ( A , F ), frames ( B , G ), frames ( C , H ), frames ( D , I ), and frames ( E , J ) show expressions of CD46, CD55, and CD59 and depositions of activated C3 (aC3) and C5b-9, respectively. Graphs ( K – O ) show results for expressions of CD46, CD55, and CD59 and deposition of aC3 and C5b-9, respectively. Original magnification of ( A – C ) and ( F – H ) is ×400, while that of ( D , E , I , J ) is ×100. Inserted frames in right upper corners of frames ( F – H ) are at double the original magnification. Scale bars of 50 μm and 200 μm are shown in the left upper corner of frames ( A , D ), respectively. * shows peritoneal cavity side. Dots in the graph show outliers. Outliers were defined as values greater than the 75th percentile plus 1.5 times the interquartile range. # p < 0.05.

Article Snippet: Monoclonal Ab (mAb) mouse anti-human CD46 (MEM 258) was purchased from BIO-RAD (Hercules, CA, USA).

Techniques: Activation Assay

Journal: International Journal of Molecular Sciences

Article Title: Peritoneal Expression of Membrane Complement Regulators Is Decreased in Peritoneal Dialysis Patients with Infected Peritonitis

doi: 10.3390/ijms24119146

Figure Lengend Snippet: Pathological changes with accumulation of inflammatory cells of peritonitis and expression of membrane complement regulators (CRegs) caused by Candida spp. or P. aeruginosa (Group P1) and Gram-positive cocci (Group P2). Graphs ( A , B ) show the tissue injury scores and the proportion of cytokeratin-positive mesothelial cells in the peritoneum, respectively. Graph ( C – E ) shows the numbers of esterase-positive neutrophils, CD3-positive pan-T cells, and CD68-positive macrophages, respectively. Graphs ( F , G ) show the scores of fibrin deposition and fibrosis in the peritoneum, respectively. Graphs ( H – J ) show CRegs CD46, CD55, and CD59, respectively. Group P1 shows cases with fungal infection or P. aeruginosa and Group P2 shows cases with Gram-positive cocci. Dots in the graph show outliers. Outliers were defined as values greater than the 75th percentile plus 1.5 times the interquartile range. # , p < 0.05.

Article Snippet: Monoclonal Ab (mAb) mouse anti-human CD46 (MEM 258) was purchased from BIO-RAD (Hercules, CA, USA).

Techniques: Expressing, Membrane, Infection